HCY32AFX2_n02_ncm8-4.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HCY32AFX2_n02_ncm8-4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18288487
Sequences flagged as poor quality	0
Sequence length	76
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	1073739	5.871119901826762	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	221999	1.2138729682778022	No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	66875	0.3656672091026447	Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	64860	0.35464934852183233	Illumina Single End PCR Primer 1 (100% over 49bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	32259	0.17638965978979015	Illumina Single End PCR Primer 1 (98% over 50bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	27427	0.1499686660793755	Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	23255	0.12715650015225427	Illumina Single End PCR Primer 1 (100% over 49bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GGTCGGA	5365	47.173943	2
GCACACG	5990	46.101105	18
AAAGGGG	173375	45.64638	67
AAGGGGG	174755	45.29269	68
GATCGAG	6610	45.218643	8
CCGTATC	207735	44.48746	49
CGCCGTA	210780	44.447533	47
CGTATCA	209250	44.23562	50
GTATCAT	206385	44.1951	51
AGGGGGG	181195	44.188988	69
GCCGTAT	210800	44.114574	48
TCGCCGT	210885	44.10675	46
GGTCGCC	214200	43.773815	44
GTCGCCG	214535	43.708725	45
CTCGGTG	209675	43.611805	38
TCTCGGT	209625	43.498657	37
GTGGTCG	214855	43.498646	42
GATCTCG	209415	43.35843	35
ATCTCGG	210820	43.358337	36
GGTGGTC	218070	43.0034	41