FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n02_ncm8-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n02_ncm8-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17397220
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC11997696.896325964723099Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2689521.5459481457382271No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC670910.3856420738485804Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC637990.36671951035855155Illumina Single End PCR Primer 1 (100% over 49bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC293750.16884881607521202Illumina Single End PCR Primer 1 (98% over 50bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC242930.13963725238860003Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC240160.1380450439782908Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG1856800.048.25729467
AAGGGGG1875250.047.7832768
GGTCGGA51650.047.509182
AGGGGGG1947950.046.4329569
GATCGAG65550.045.5446628
AAAAGGG1981850.045.4896466
CGATCGG21600.042.972851
GCACACG52950.042.69998618
CCGTATC2411650.041.8225749
GTATCAT2378350.041.81067751
CGCCGTA2449200.041.69009447
GAGCACA111150.041.6592616
CGTATCA2430700.041.60854750
GCCGTAT2443000.041.5022148
TCGCCGT2447150.041.43325846
CTCGGTG2412900.041.26856238
GGTCGCC2480600.041.22727244
GTCGCCG2481650.041.18021845
TCTCGGT2413000.041.11746237
GATCTCG2403050.041.0561335
Produced by FastQC (version 0.11.5)