HCY32AFX2_n02_ncm8-2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HCY32AFX2_n02_ncm8-2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16788451
Sequences flagged as poor quality	0
Sequence length	76
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	1353169	8.06011823246826	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	273752	1.6305971289429857	No Hit
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	68322	0.40695833105746326	Illumina Single End PCR Primer 1 (100% over 49bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	67673	0.40309257834448214	Illumina Single End PCR Primer 1 (100% over 49bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	31248	0.18612795188787817	Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	24864	0.14810181117960197	Illumina Single End PCR Primer 1 (100% over 49bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	24746	0.14739894704996906	Illumina Single End PCR Primer 1 (98% over 50bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GATCGAG	8480	50.187893	8
GCACACG	6420	47.70175	18
GAGCACA	12685	46.62915	16
AAAGGGG	205350	46.164246	67
AAGGGGG	206695	45.872917	68
GGTCGGA	5460	45.83849	2
GTGACTG	7140	44.936996	1
AGGGGGG	213285	44.84118	69
AGCACAC	11095	44.66807	17
AGAGCAC	14155	44.110935	15
AAAAGGG	217835	43.8076	66
AGATAGA	21160	43.79894	7
GATAGAT	21095	43.46934	8
AGTTCGG	4785	42.750153	1
GTATCAT	259300	42.55815	51
CCGTATC	262820	42.543472	49
CGCCGTA	267360	42.419296	47
CGTATCA	264545	42.381165	50
TCGCCGT	267025	42.161877	46
GCCGTAT	266770	42.160194	48