FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n02_ncm8-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n02_ncm8-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18053705
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5876403.2549551463259205Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1547870.8573697199549899No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC402600.22300131745810625Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC396450.21959481447160015Illumina Single End PCR Primer 1 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG979050.044.925767
AAGGGGG992400.044.34177868
AGGGGGG1030650.043.14439469
CGCCGTA1201000.041.95851547
CCGTATC1190300.041.82700749
GCACACG49950.041.62093718
TCGCCGT1203300.041.6078146
GGTCGCC1217000.041.4500244
GCCGTAT1209000.041.3855948
GTCGCCG1219650.041.35421845
CTCGGTG1191350.041.2437138
CGTATCA1213650.041.1924250
GTGGTCG1226000.040.97730642
TCTCGGT1198350.040.84799637
GATCTCG1193000.040.81408335
ATCTCGG1202400.040.81228636
AAAAGGG1086150.040.7793766
GTATCAT1213750.040.5863651
TCGGTGG1232000.040.38854239
GGTGGTC1254100.040.29636441
Produced by FastQC (version 0.11.5)