FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n02_ncm8-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n02_ncm8-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4983
Sequences flagged as poor quality0
Sequence length76
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3236.482038932370059Illumina Single End PCR Primer 1 (100% over 50bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC350.7023881196066627Illumina Single End PCR Primer 1 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240.481637567730283No Hit
AAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC230.46156933574152115Illumina Single End PCR Primer 1 (98% over 50bp)
AGATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC220.44150110375275936Illumina Single End PCR Primer 1 (98% over 50bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC120.2408187838651415Illumina Single End PCR Primer 1 (100% over 38bp)
AGAACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC120.2408187838651415Illumina Single End PCR Primer 1 (98% over 50bp)
ATATCTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC110.22075055187637968Illumina Single End PCR Primer 1 (97% over 44bp)
TTTCTTCTTTATTTGGCTAGCCAGTAATTTTTTTTACTGCATATAGTAAC100.2006823198876179No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC100.2006823198876179Illumina Single End PCR Primer 1 (98% over 50bp)
AGCTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC70.14047762392133253Illumina Single End PCR Primer 1 (98% over 50bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC60.12040939193257075Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC50.10034115994380895Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG207.175826E-570.0000138
GATCGGA409.458745E-1170.000012
GTATCAT650.070.0000151
CCGTATC650.070.0000149
CTGAAGA207.175826E-570.000015
CGTATCA650.070.0000150
TATCATT650.070.0000152
ACGAAAA207.175826E-570.000014
ACGTTGT150.00209596470.039
GTACCCC150.00209596470.044
ATCGGAA500.070.03
GATCTCG550.070.035
TCTGAAG150.00209596470.04
ATCATTA700.070.053
CTCGGTG550.070.038
CGTTGTA150.00209596470.040
AAACGTT150.00209596470.037
AGATCTC550.070.034
ACGGTGG150.00209596470.039
TGTACCC150.00209596470.043
Produced by FastQC (version 0.11.5)