FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n02_ncm8-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n02_ncm8-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12428
Sequences flagged as poor quality0
Sequence length76
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC7876.332475056324428Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7786.260057933698102No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG390.3138075313807531No Hit
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC280.22529771483746378Illumina Single End PCR Primer 1 (100% over 49bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC180.14483424525265529Illumina Single End PCR Primer 1 (100% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170.13678789829417443No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC150.12069520437721275Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC950.070.0000120
CACGTCT650.070.0000121
GGAGATC207.623906E-570.05
ACACGTA150.002173221770.020
CACACGT1100.070.019
ACGTCTG207.623906E-570.022
ACGTCTC150.002173221770.022
ACGTCTA150.002173221770.022
AGAAGAT207.623906E-570.04
CACGTCA252.6786856E-670.021
GGATCGG207.623906E-570.07
GCGCGCG252.6786856E-670.018
GAAGATC309.4323696E-870.05
CCGCAGA150.002173221770.03
GAGCACA1150.070.016
AGAGCGC207.623906E-570.015
AGAGCAC1250.070.015
GCACGCG150.002173221770.018
ATCGGAG207.623906E-570.09
GCAGATC207.623906E-570.05
Produced by FastQC (version 0.11.5)