FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n02_ncm8-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n02_ncm8-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC54126.729249011857707Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG472.322134387351779No Hit
ATATCTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC422.075098814229249Illumina Single End PCR Primer 1 (97% over 44bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC160.7905138339920948Illumina Single End PCR Primer 1 (100% over 38bp)
AGATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC90.4446640316205534Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC60.2964426877470355Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAA60.2964426877470355Illumina Single End PCR Primer 1 (100% over 26bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC50.24703557312252966Illumina Single End PCR Primer 1 (100% over 49bp)
ATATCTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC50.24703557312252966Illumina Single End PCR Primer 1 (96% over 31bp)
AGAACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC50.24703557312252966Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC50.24703557312252966Illumina Single End PCR Primer 1 (100% over 49bp)
GTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAAA40.1976284584980237Illumina Single End PCR Primer 1 (100% over 27bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC40.1976284584980237Illumina Single End PCR Primer 1 (98% over 50bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC40.1976284584980237Illumina Single End PCR Primer 1 (97% over 38bp)
ATAACTGAATACAGACGTGTACTCAAACAATCTAGATCTCAATAGACGCC30.14822134387351776No Hit
ATATCGGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC30.14822134387351776Illumina Single End PCR Primer 1 (100% over 38bp)
AGATCTGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC30.14822134387351776Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAAGA305.9568265E-870.000015
TGAAGAT305.9568265E-870.000016
TCGTCGT150.001913255770.0000112
ATCGTCG150.001913255770.0000111
GATCGTC206.1526895E-570.010
TCTGAAG206.1526895E-570.04
ATATCTG206.1526895E-570.01
AGATCGT206.1526895E-570.09
TCATTAA900.066.11111554
CGGTGGT950.062.63158440
TATCATT950.062.63158452
ATCATTA950.062.63158453
CTCGGTG950.062.63158438
GGTGGTC950.062.63158441
GTAGATC900.062.22222532
ATCTCGG900.062.22222536
TCTCGGT900.062.22222537
GATCTCG900.062.22222535
AGATCTC900.062.22222534
TAGATCT900.062.22222533
Produced by FastQC (version 0.11.5)