FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10276998
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA8038597.8219242623186265TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG833010.8105577134490052No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC457100.4447796915013509TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC326150.3173592132644183TruSeq Adapter, Index 14 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG326100.31731056092450344TruSeq Adapter, Index 14 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG280330.2727742089664706TruSeq Adapter, Index 11 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG267340.26013433105659844TruSeq Adapter, Index 9 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG204040.1985404687244271TruSeq Adapter, Index 12 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCACTCACAGATCGGAAGAGCACA201760.19632192202431098TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG183590.17864166169926277TruSeq Adapter, Index 7 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCCACAGATCGGAAGAGCACA177950.17315367775687024TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAATCACAGATCGGAAGAGCACA172310.1676656938144777TruSeq Adapter, Index 13 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC162110.15774061647185297TruSeq Adapter, Index 14 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG146750.14279461765001805TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG145660.1417339966398748TruSeq Adapter, Index 20 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCGGTCACAGATCGGAAGAGCACA116990.11383674493271284TruSeq Adapter, Index 13 (97% over 35bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC116480.11334049106558161TruSeq Adapter, Index 14 (97% over 36bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGT115180.1120755302277961TruSeq Adapter, Index 14 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT110580.10759951495563197TruSeq Adapter, Index 14 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGACACAGATCGGAAGAGCACA109130.10618859709810201TruSeq Adapter, Index 13 (97% over 35bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC109110.10616913616213607TruSeq Adapter, Index 14 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG86250.054.36481
AATCGGA113500.054.3520851
TGACTGG88300.052.8952562
AAAAAGG466300.049.3119870
GCCGGCT53400.048.2976850
CACGCGT9600.046.3640547
ACTGGAG99900.046.1642844
GGGCTCC11800.044.3043852
GACTGGA105400.044.247333
GCGGGCT10750.043.38205750
CCGGCTT61550.042.8178951
TCACAGA1525800.042.49308431
TGCCGGC72800.042.4665849
GCTTGAA533550.041.99115460
CACAGAT1585750.041.86218332
ATGCCGG68250.041.7233648
CGGCTTC61400.041.30693452
CGCGTCT13350.041.28705649
TGCTTGA529950.041.13529659
ACAGATC1612300.040.97525433
Produced by FastQC (version 0.11.5)