FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_ncm8-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_ncm8-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG562.457218078104432TruSeq Adapter, Index 20 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG241.0530934620447565TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGGATG150.6581834137779727TruSeq Adapter, Index 20 (97% over 42bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120.5265467310223783No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATG120.5265467310223783TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCACCCACAGATCGGAAGAGCACA90.39491004826678366Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGATCGGAAGAGCACAC90.39491004826678366Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGGATG70.307152259763054TruSeq Adapter, Index 20 (97% over 45bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT70.307152259763054TruSeq Adapter, Index 14 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAACCACAGATCGGAAGAGCACA70.307152259763054Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC60.26327336551118913TruSeq Adapter, Index 14 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGTCGTATG60.26327336551118913TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCGTCGAGGAAG40.17551557700745943TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCGTCGCGGATG40.17551557700745943TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCGTCGCGGAAG40.17551557700745943TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGGAAG40.17551557700745943TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTAACAGATCGGAAGAGCACA30.13163668275559456TruSeq Adapter, Index 13 (97% over 35bp)
AGTCGGAAGAGCACACTCTGAACTCCAGTCACAGATCGGAAGAGCACACG30.13163668275559456No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC30.13163668275559456TruSeq Adapter, Index 10 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGCCATCTCGTATG30.13163668275559456TruSeq Adapter, Index 20 (97% over 39bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCC30.13163668275559456TruSeq Adapter, Index 20 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG30.13163668275559456TruSeq Adapter, Index 4 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCTAGTCACAGATCGGAAGAGCACA30.13163668275559456TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTAAG30.13163668275559456TruSeq Adapter, Index 20 (97% over 47bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC30.13163668275559456TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCGTCGCGGACG30.13163668275559456TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGAGGATG30.13163668275559456TruSeq Adapter, Index 20 (97% over 42bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.13163668275559456No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTTT206.3353546E-570.0000111
GGCCATC206.3353546E-570.0000137
AAAAAGG206.3353546E-570.0000170
CAACTTT150.001946575970.09
CATCTCG150.001946575970.040
AACTTTT150.001946575970.010
CCATCTC150.001946575970.039
GCCATCT150.001946575970.038
AATCGGA150.001946575970.01
CTTTTTT251.9130488E-455.99999612
GCTTGAA200.006088450652.50000460
TTGAAAA200.006088450652.50000462
CTTGAAA200.006088450652.50000461
AAAAAAG304.7101782E-446.66666469
GATCGGA752.6921043E-1042.02
AGATCGG752.6921043E-1042.01
ACACGTC859.222276E-1037.05882314
ACGTCTG859.222276E-1037.05882316
CACGTCT859.222276E-1037.05882315
GTCTGAA859.222276E-1037.05882318
Produced by FastQC (version 0.11.5)