FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_ncm8-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_ncm8-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16090088
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG7337434.5602174456721425TruSeq Adapter, Index 20 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGTCGTATG1005100.624670293910139TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATG804660.5000967054996841TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGTATG393790.244740737278752TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC364310.2264188983926005TruSeq Adapter, Index 20 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344920.2143680009705354No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGGATG261430.162478912483263TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGGATG238600.1482900528573865TruSeq Adapter, Index 20 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGGATG226170.14056479989419574TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGTCGTATGC213360.1326033766875607TruSeq Adapter, Index 20 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA1092600.052.89565359
AAAAAGG1270550.052.5304970
CTGCTTG1145850.052.34649358
CTTCTGC1422550.051.61672655
CTCGTAT1116900.050.35946743
TCTGCTT1213900.050.17892557
GCTTGAA1193400.050.03203660
TTCTGCT1172350.050.03178456
GTCTTCT1240600.049.6375153
TCTCGTA1141100.049.62577442
GCCGTCT1262900.049.45939350
TCTTCTG1276500.048.68842354
CGTCTTC1298150.048.64479452
CATCTCG1231250.048.3998940
CCGTCTT1322950.048.2170351
ATCTCGT1210700.047.80782341
TGCCGTC1357550.047.3980349
ATGCCGT1339600.047.35647648
CTTGAAA1287800.047.01949761
GGCTTCT271500.046.5618753
Produced by FastQC (version 0.11.5)