FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_ncm8-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_ncm8-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15535705
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG12472808.02847376414524TruSeq Adapter, Index 13 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC1252460.806181631280975TruSeq Adapter, Index 13 (98% over 50bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTAT662020.4261280707891917TruSeq Adapter, Index 13 (97% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTAT574510.36979976125962744TruSeq Adapter, Index 13 (97% over 48bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC461710.29719282131065183TruSeq Adapter, Index 13 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG448990.28900523021002267No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC417470.26871648245123086TruSeq Adapter, Index 13 (98% over 50bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCG312240.2009821890928027TruSeq Adapter, Index 13 (98% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTAT225900.1454069834616453TruSeq Adapter, Index 13 (97% over 48bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC217060.13971686511812628TruSeq Adapter, Index 13 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATATCGTATG193310.12442949965901129TruSeq Adapter, Index 13 (97% over 41bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTAT159650.10276327981253507TruSeq Adapter, Index 13 (97% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG1460050.051.36732570
AGTCGGA53250.044.3900681
CCGCGTG34550.042.14034758
GCGTGGA76050.041.8788760
TGCTTGA2137000.039.84160259
AATCGGA111050.039.5124661
TCCGCGT36100.039.45844757
CTTGAAA2225950.039.05296361
CTGCTTG2184650.039.0158658
GCTTGAA2226250.038.78359260
CTTCTGC2363350.038.5003555
GTCTTCT2352750.038.25592453
AGATCGG2698200.038.1635441
TTCTGCT2222250.038.1084656
TCTGCTT2248050.038.01983657
TCTTCTG2376150.037.8173254
TTGAAAA2317250.037.7619762
GCCGTCT2441500.037.69672850
TGAAAAA2578050.037.3100363
CGTCTTC2464750.037.30421452
Produced by FastQC (version 0.11.5)