FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_ncm8-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_ncm8-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14984213
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG10930447.294637362669631TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC737270.4920311797489798TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG448120.2990614188412832No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT439050.2930083815546402TruSeq Adapter, Index 12 (100% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT436810.291513474881864TruSeq Adapter, Index 12 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATG378500.25259918555615835TruSeq Adapter, Index 12 (97% over 49bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC300060.2002507572469772TruSeq Adapter, Index 12 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATG270800.180723538833838TruSeq Adapter, Index 12 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC222220.14830275036800397TruSeq Adapter, Index 12 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC202840.1353691381722884TruSeq Adapter, Index 12 (100% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT175470.1171032472643041TruSeq Adapter, Index 12 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG1316750.053.61894270
GCGTGGA121700.047.2788260
TGCTTGA1635900.044.6542459
CTGCTTG1676900.043.81916858
CTCGTAT1910250.043.42376743
CTTCTGC1938500.043.2748155
CCGCGTG45350.043.06379758
TCTCGTA1942250.042.9822342
AGTCGGA49450.042.8458061
GTCTTCT1864600.042.81823353
TTCTGCT1704950.042.7287556
GCTTGAA1751300.042.45322860
ATCTCGT1996250.042.2876441
TCTGCTT1751650.042.25892657
TCTTCTG1894800.042.17272654
CGCGTGG34150.042.12176559
AATCTCG2032050.041.80270440
CTTGAAA1802050.041.64996361
GCCGTCT1960950.041.58357650
TAATCTC2051200.041.23498539
Produced by FastQC (version 0.11.5)