FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_ncm8-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_ncm8-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13157608
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG10669718.10915631473441TruSeq Adapter, Index 11 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC800140.6081196521434595TruSeq Adapter, Index 11 (100% over 50bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT679390.5163476522480378TruSeq Adapter, Index 11 (100% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT667810.5075466604568247TruSeq Adapter, Index 11 (100% over 48bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG456900.34725156730615475No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC350030.2660285972951923TruSeq Adapter, Index 11 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC330550.2512234746619599TruSeq Adapter, Index 11 (100% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT236060.1794095096920352TruSeq Adapter, Index 11 (100% over 48bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG186560.14178868985912943TruSeq Adapter, Index 11 (100% over 50bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC157990.12007501667476338TruSeq Adapter, Index 11 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG1139100.052.77577670
CCGCGTG18200.042.69045358
TGCTTGA1765100.041.53380259
CTTGAAA1808500.041.4853461
GCTTGAA1818050.041.08454560
CGCGTGA14050.040.85231859
AGTCGGA34600.040.69051
CTGCTTG1804200.040.61817658
TTGAAAA1884300.039.97252362
TGAAAAA2025850.039.94026663
AGATCGG2100650.039.901251
CTTCTGC1922700.039.6784155
TCTGCTT1862000.039.46257457
TTCTGCT1848550.039.41269356
GCCGTCT1980250.039.3083850
GTCTTCT1931900.039.25591353
CCGTCTT2006550.038.90653651
TCTTCTG1953500.038.84141554
TGCCGTC2062150.038.82168249
ATGCCGT2066950.038.75184648
Produced by FastQC (version 0.11.5)