FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_ncm8-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_ncm8-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18288487
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG6659603.641416591760707TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATG1618390.8849228479097259TruSeq Adapter, Index 10 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG505900.2766221175103222No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATG480640.2628101493578993TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATG410610.22451829940880294TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATG371370.2030621778608586TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATG356490.19492591158579714TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTG339290.1855210876656992TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTATG325540.17800269645050462TruSeq Adapter, Index 10 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC265390.14511315233458078TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATT219360.11994431250655126TruSeq Adapter, Index 10 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTG190670.10425684749099257TruSeq Adapter, Index 10 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG1025800.056.20705458
TGCTTGA993700.055.47627659
CTTCTGC1319850.054.23811355
GCCGTCT1028900.054.19375650
ATGCCGT1040000.053.79033748
AAAAAGG1219000.053.1353170
TCTGCTT1109700.052.88786757
TGCCGTC1092600.052.68706549
GTCTTCT1063800.052.61322453
TTCTGCT1074800.052.53743756
CCGTCTT1100650.052.2953651
CGTCTTC1091300.052.24952752
GCTTGAA1092500.051.79197360
TATGCCG1277350.051.2781147
TCTTCTG1112450.050.99173754
GTATGCC1317800.050.66274646
TCGTATG991450.050.33842544
CGTATGC1231550.049.91967445
CTCGTAT1029350.049.88242343
TCTCGTA1058200.049.07148742
Produced by FastQC (version 0.11.5)