FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_ncm8-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_ncm8-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17397220
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG13497757.758567173375976TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1141340.6560473454954298TruSeq Adapter, Index 9 (100% over 50bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT763110.43863904692818734TruSeq Adapter, Index 9 (100% over 48bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT750610.43145399092498693TruSeq Adapter, Index 9 (100% over 48bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC512250.29444359501115697TruSeq Adapter, Index 9 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG465790.2677381788584613No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC458880.26376627989989204TruSeq Adapter, Index 9 (100% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT283770.16311226736225673TruSeq Adapter, Index 9 (100% over 48bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG259990.14944341682176807TruSeq Adapter, Index 9 (100% over 50bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC226770.1303484119876624TruSeq Adapter, Index 9 (100% over 48bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC188580.1083966288866842TruSeq Adapter, Index 9 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG1471900.050.78970370
AGTCGGA53200.041.4717751
AATCGGA107250.038.824711
CTTGAAA2703700.037.1632461
GCTTGAA2719150.036.7963460
TGCTTGA2704350.036.57969759
AGATCGG2919000.036.3127331
TTGAAAA2793650.036.1295262
TGAAAAA2865000.036.12874263
CTGCTTG2753550.035.92609458
GCCGTCT2866550.035.28263550
GAAAAAA2946950.035.2701464
GATCTCG2847650.035.2156940
CTCGTAT2877150.035.2026243
ATCTCGT2862500.035.1662741
TGCCGTC2901750.035.14782349
CCGTCTT2887100.035.12120451
CTTCTGC2859850.035.08871555
TCTGCTT2825100.035.07691657
TCTCGTA2882000.035.04612742
Produced by FastQC (version 0.11.5)