FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_ncm8-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_ncm8-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16788451
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG9633455.738141058993471TruSeq Adapter, Index 7 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATG1440630.8581077551466779TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGATG469810.27984118368037647TruSeq Adapter, Index 7 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC467790.2786379755940557TruSeq Adapter, Index 7 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG451370.2688574425359433No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATG442290.2634489626231747TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTG310040.18467457182321348TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGATG270310.1610094939670134TruSeq Adapter, Index 7 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATG244720.1457668727150587TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGTATG236000.1405728259265849TruSeq Adapter, Index 7 (97% over 42bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC211490.12597350404751456TruSeq Adapter, Index 7 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC178970.10660304515288516TruSeq Adapter, Index 7 (100% over 50bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT174640.10402389118567282TruSeq Adapter, Index 7 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG1456250.054.7480470
TGCTTGA1289500.053.6283459
CTGCTTG1344150.053.39035458
CTTCTGC1673500.052.29625355
TTCTGCT1353000.051.6599656
TCTGCTT1406000.051.6391357
GTCTTCT1419600.051.1199253
GCCGTCT1465550.050.3720750
GCTTGAA1424150.050.05699560
TCTTCTG1462300.050.02448754
ATGCCGT1487550.049.96354348
CGTCTTC1501450.049.60355852
CCGTCTT1527400.049.16638651
CTCGTAT1487400.048.99193643
TGCCGTC1566750.048.70221749
TCGTATG1479500.048.5769844
TCTCGTA1515600.048.4013542
GATGCCG182050.048.27342247
CTTGAAA1518050.047.57855661
TATGCCG1779700.047.3506247
Produced by FastQC (version 0.11.5)