FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_ncm8-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_ncm8-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18053705
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG6513993.6081181120440378TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC566100.31356444563595115TruSeq Adapter, Index 4 (100% over 50bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT432220.23940792208579903TruSeq Adapter, Index 4 (100% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT427100.23657193911166713TruSeq Adapter, Index 4 (100% over 48bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC292670.1621107689529656TruSeq Adapter, Index 4 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG277720.15382992022967032No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC207550.11496255200802274TruSeq Adapter, Index 4 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG868450.044.6203770
TGCTTGA1233300.036.90003259
CTGCTTG1246900.036.50036658
GCTTGAA1270000.036.39037760
GCCGTCT1325500.036.2608250
TGCCGTC1374550.035.79183649
CTCGTAT1370500.035.67543443
CCGTCTT1355050.035.55529851
ATGCCGT1385950.035.54794348
ATCTCGT1377300.035.4840541
TATGCCG1412700.035.24396547
TCTCGTA1385700.035.21843342
GTATGCC1418000.035.18134346
CTTCTGC1346850.035.1299355
CTTGAAA1327100.035.07781661
CGTCTTC1378750.034.8806652
AATCTCG1398550.034.84229340
GTCTTCT1355850.034.7857453
CGTATGC1431450.034.78476345
TCTGCTT1313450.034.76820857
Produced by FastQC (version 0.11.5)