FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_ncm8-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_ncm8-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4983
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG2114.234396949628738TruSeq Adapter, Index 20 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG641.2843668472807546TruSeq Adapter, Index 13 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG571.1438892233594222No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCGGAAGAGCACAC390.78266104756171TruSeq Adapter, Index 13 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATG270.5418422636965683TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGTCGTATG170.3411599438089504TruSeq Adapter, Index 20 (97% over 41bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160.32109171182018864No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGGATG130.2608870158539033TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGGATG120.2408187838651415TruSeq Adapter, Index 20 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACATCTCGTATG110.22075055187637968TruSeq Adapter, Index 13 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAAATCTCGTATG100.2006823198876179TruSeq Adapter, Index 13 (97% over 39bp)
GCACAGGAATGCGAACAGGATTGATTCCGATGATTTAACATCTAAGCAAG100.2006823198876179No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCCATCTCGTATG80.16054585591009432TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCATATCTCGTATG80.16054585591009432TruSeq Adapter, Index 13 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGGTG70.14047762392133253TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC70.14047762392133253TruSeq Adapter, Index 20 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCCCATCTCGTATG60.12040939193257075TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACAGATCGGAAG60.12040939193257075TruSeq Adapter, Index 13 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTTATCTCGTATG60.12040939193257075TruSeq Adapter, Index 9 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCTTATCTCGTATG60.12040939193257075TruSeq Adapter, Index 4 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCCCATCTCGTATG60.12040939193257075TruSeq Adapter, Index 9 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCAAATCTCGTATG60.12040939193257075TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTTTG50.10034115994380895TruSeq Adapter, Index 20 (97% over 46bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA207.175826E-570.0000142
GCGTATG207.175826E-570.0000144
CGCGTAT207.175826E-570.0000143
AGTCGGA150.00209596470.01
GTCGGAA150.00209596470.02
TGCTTGA600.064.16666459
TTCTGCT509.094947E-1262.99999656
GCCGTCT509.094947E-1262.99999650
CTTCTGC509.094947E-1262.99999655
CATCTCG352.4125438E-760.040
GCCATCT352.4125438E-760.038
GGCCATC650.059.23077437
AAAAAGG650.059.23077470
TGCCGTC600.058.33333249
TCTGCTT600.058.33333257
CCATCGC307.2175208E-658.33333239
ATGCCGT600.058.33333248
GCCATCG307.2175208E-658.33333238
CTGCTTG600.058.33333258
CATCGCG307.2175208E-658.33333240
Produced by FastQC (version 0.11.5)