FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_ncm8-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_ncm8-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12428
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4723.797875764402961No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCGGATCTCGTATG570.45864177663340844TruSeq Adapter, Index 9 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGGATCTCGTATG280.22529771483746378TruSeq Adapter, Index 10 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGGGATCTCGTATG210.16897328612809784TruSeq Adapter, Index 20 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG180.14483424525265529TruSeq Adapter, Index 9 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGGGATCTCGTATG180.14483424525265529TruSeq Adapter, Index 20 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGGGATCTCGTATG170.13678789829417443TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCGGATCGCGTATG150.12069520437721275TruSeq Adapter, Index 9 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCGGATCTCGTATG140.11264885741873189TruSeq Adapter, Index 18 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG130.10460251046025104TruSeq Adapter, Index 10 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATC453.6379788E-1270.0000137
ACACGTT207.623906E-570.014
GAGCGGA252.6786856E-670.035
CACGTTG150.002173221770.015
GGATCTC353.3269316E-970.039
CACGAGC309.4323696E-870.032
CGGATCT309.4323696E-870.038
CGGATCG150.002173221770.038
TCACGAG309.4323696E-870.031
ACGAGCG252.6786856E-670.033
GCCGGCT150.002173221770.050
CGAGCGG252.6786856E-670.034
CACTAGG150.002173221770.032
CGCGTAT207.623906E-570.043
TGCGGAT150.002173221770.036
CCGGCTT207.623906E-570.051
AGCGGAT352.7414353E-760.036
TCGCGTA252.299684E-455.99999642
ATGCCGG252.299684E-455.99999648
ATCGCGT252.299684E-455.99999641
Produced by FastQC (version 0.11.5)