FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n01_ncm8-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n01_ncm8-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1135.58300395256917TruSeq Adapter, Index 9 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG482.371541501976284No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCGATCTCGTATG341.6798418972332017TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAGATCTCGTATG291.4328063241106719TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGAGATCTCGTATG261.2845849802371543TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGAGATCTCGTATG251.2351778656126482TruSeq Adapter, Index 12 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCCGATCTCGTATG231.1363636363636365TruSeq Adapter, Index 9 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCGATCTCGTATG221.0869565217391304TruSeq Adapter, Index 20 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCAGATCTCGTATG221.0869565217391304TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCGATCTCGTATG200.9881422924901186TruSeq Adapter, Index 21 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCACATCTCGTATG150.7411067193675889TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGCGATCTCGTATG150.7411067193675889TruSeq Adapter, Index 20 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGAGATCTCGTATG130.6422924901185771TruSeq Adapter, Index 19 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG130.6422924901185771TruSeq Adapter, Index 20 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGACATCTCGTATG130.6422924901185771TruSeq Adapter, Index 12 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTAACAGATCGGAAGAGCACA120.592885375494071TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCAGATCTCGTATG110.5434782608695652TruSeq Adapter, Index 19 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGAGATCTCGTATG110.5434782608695652TruSeq Adapter, Index 20 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCACCCACAGATCGGAAGAGCACA100.4940711462450593Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGATCGGAAGAGCACAC100.4940711462450593Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCCGATCTCGTATG90.4446640316205534TruSeq Adapter, Index 20 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCAGATCTCGTATG80.3952569169960474TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCGATCTCGTATG80.3952569169960474TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGACATCTCGTATG70.3458498023715415TruSeq Adapter, Index 20 (97% over 39bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT70.3458498023715415TruSeq Adapter, Index 14 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCCATCTCGTATG60.2964426877470355TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCCGATCTCGTATG50.24703557312252966TruSeq Adapter, Index 21 (97% over 39bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTC50.24703557312252966TruSeq Adapter, Index 14 (96% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTTACAGATCGGAAGAGCACA50.24703557312252966TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCAAGTCACGATCAGATCTCGTATG40.1976284584980237TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCATCTCGTATG40.1976284584980237TruSeq Adapter, Index 9 (97% over 36bp)
GAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCT40.1976284584980237TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACAGATCTCGTATG40.1976284584980237TruSeq Adapter, Index 4 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGCCATCTCGTATG40.1976284584980237TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTACAGTCACGATCAGATCTCGTATG30.14822134387351776TruSeq Adapter, Index 9 (97% over 49bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT30.14822134387351776TruSeq Adapter, Index 9 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGACACAGATCGGAAGAGCACA30.14822134387351776TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA30.14822134387351776TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGGATG30.14822134387351776TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACCGTCTGAACTCCAGTCACGATCAGATCTCGTATGC30.14822134387351776TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGACATCTCGTATG30.14822134387351776TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCTGTCTCTCAATT30.14822134387351776TruSeq Adapter, Index 21 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTTGCAGATCGGAAG30.14822134387351776TruSeq Adapter, Index 11 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCGCAGATCGGAAGAGCACA30.14822134387351776TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTTCAGTCACGATCAGATCTCGTATG30.14822134387351776TruSeq Adapter, Index 9 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC30.14822134387351776TruSeq Adapter, Index 21 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCCGATCTCGTATG30.14822134387351776TruSeq Adapter, Index 9 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG30.14822134387351776TruSeq Adapter, Index 9 (97% over 49bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT30.14822134387351776TruSeq Adapter, Index 9 (100% over 48bp)
AGATCGGAAGAGCACATGTCTGAACTCCAGTCACGATCAGATCTCGTATG30.14822134387351776TruSeq Adapter, Index 9 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC30.14822134387351776TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCACATCTCGTATG30.14822134387351776TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGTGCTTTCATCT30.14822134387351776TruSeq Adapter, Index 21 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC150.001913255770.0000132
CCGATCT150.001913255770.0000138
GAGATCT150.001913255770.0000138
TCACGAG206.1526895E-570.031
CGATCTC251.9356794E-670.039
GAAAAAA950.062.63158464
TTGAAAA800.061.2562
CTTGAAA800.061.2561
TTCTGCT750.060.66666856
TCTGCTT750.060.66666857
GCTTGAA750.060.66666860
TGCTTGA700.060.00000459
CTGCTTG700.060.00000458
TGAAAAA1000.059.49999663
GTATGCC950.058.94737246
ATCTCGT950.058.94737241
TATGCCG950.058.94737247
TCTCGTA950.058.94737242
GATCTCG950.058.94737240
CGTATGC950.058.94737245
Produced by FastQC (version 0.11.5)