FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_wt4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_wt4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8947653
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG219550.24537160750422482No Hit
CTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCC214690.239940015554917No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA195500.21849305063573657No Hit
CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGG174560.19509026557020034No Hit
CTTTTCTGCTTAACGGCCCGCCAACCCTGGAAACGGTTCAGCCGGAGGTA167370.18705463879745896No Hit
CGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGC144760.16178544250654334No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTTGGGAAG127840.14287545572006424No Hit
ATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTGGTCATCGCG123360.13786855614539367No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG121550.13584567930830577No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC118500.13243696419608583No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT115640.1292405952711845No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA115280.12883825512679134No Hit
CTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCC114370.1278212286506864No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGC111560.12468074030139524No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGA109340.122199642744304No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTC107800.120478521015511No Hit
CTCAAACTTCCGTGGCCTAAACGGCCATAGTCCCTCTAAGAAGCTAGCTG107390.12002030029550767No Hit
CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCC101340.11325875064667795No Hit
CCCGGATCGGCCCGAGGGACTCGGGCCTTAGAGCCAAAAGGAGGGGCCAG101200.11310228503496951No Hit
GCCAGAGGCGCGCGGTGACCGGCTGCGCCGACGACGGCTGCCGGGGGCGC99270.11094529481641723No Hit
GTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCCCGA98640.11024119956372917No Hit
CGTTATTTTACTTATTCCGTGAGTCGGAAGCGGGGCCTGGCCCCTCCTTT96920.10831890776273956No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA92400.10326730372758085No Hit
GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGAGTCGGAA91210.10193734602805897No Hit
ATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGC90190.10079738228561165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT5050.034.6583561
TGTTACG16850.030.5341663
GTTACGA18900.027.4074464
TTACGAC19250.026.9091265
CGCGGTT8300.024.0395361
TACGACT22250.023.4382346
CGCATAG38400.022.51506665
CATGCGT76050.022.1302287
ACATGCG77600.022.010346
ATGCGTG76800.021.8685518
GTCCCCC34250.021.76864269
TCAGGCG17050.021.5544032
TGCGTGC79950.021.4008189
GAACCGC25100.021.05815968
GCATAGC42050.020.97716966
TCCCCCG35800.020.92403870
TAGTCCC41150.020.83714328
CTTGTTA26100.020.7883741
CCCCCTT15700.020.735411
TAAGTCG47000.020.6215789
Produced by FastQC (version 0.11.5)