FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_wt1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_wt1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8044889
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGC139960.17397381119863806No Hit
GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG97270.12090906412754732No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTTGGGAAG95790.11906938678706443No Hit
CTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCC85500.10627865716978818No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA83380.10364344368206946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA12100.023.1414174
TGTTACG12300.022.7651333
TTACGAC12850.022.0629985
CGGAATA34700.017.95594468
CGCGGTT11800.017.7999741
ACATGCG46550.017.5193846
TACGACT16150.017.3380456
TAAGTCG41400.017.241819
CATGCGT46700.017.1583047
GACTGTC34050.017.063824
TTCCGAC36700.016.97773658
ACTCACG36700.016.9772163
GACTCAC36400.016.82874562
ATGCGTG48100.016.7315378
CGCATAG20800.016.66014565
GAATAAG37850.016.5539770
TAGCGAC34700.016.14168248
TGCGTGC51050.016.107399
ACGTTAA18100.016.04876532
GGAATAA39800.016.00655769
Produced by FastQC (version 0.11.5)