FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10680588
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG727781268.1405555574281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG12600.042.99333615
CACGTCT13200.041.03698714
ACACGTC13400.040.685113
CTCCAGT12650.038.43387624
CGTCTGA14400.038.1054716
CTGAACT14600.037.8354419
CACACGT14700.037.561712
CAGTCAC9650.036.6174227
CCAGTCA10800.036.6036126
AACTCCA16350.033.79638322
GCACACG16800.033.49037611
TGAACTC16750.032.98667520
AGCACAC17500.032.35045610
GAACTCC17300.031.33302121
AGAGCAC23050.025.0158028
ATACGGC2001.8189894E-1224.486615
TCCAGTC19950.023.31970625
TCTGAAC24250.023.20614218
ATCGGAA26850.022.1541612
TGATACG2259.094947E-1221.7671993
Produced by FastQC (version 0.11.5)