FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_ox7.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_ox7.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7535163
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGC146430.1943289083461101No Hit
GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG117750.1562673561275317No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTTGGGAAG99660.13225991262564593No Hit
CTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCC94810.12582342279788772No Hit
ATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTGGTCATCGCG90000.1194400174223172No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA89180.11835178615246943No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC86700.11506055011683224No Hit
GCCAGAGGCGCGCGGTGACCGGCTGCGCCGACGACGGCTGCCGGGGGCGC86390.1146491456123776No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG84100.11161006072463198No Hit
GTCGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGC83400.11068108281134727No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA81650.10835863802813556No Hit
GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGAGTCGGAA80400.10669974889727003No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA77220.10247953494834816No Hit
CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGG75860.10067466357396648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT9200.023.9727761
TGTTACG11350.020.6625543
TCGGATG32200.020.1110762
TTACGAC12300.019.635825
GTTACGA12350.019.5563224
GTCAGGC17950.018.5278381
GTGCGAC18750.018.4815753
TCAGGCG16250.017.879042
ACATGCG46200.017.6530196
CATGCGT46400.017.3449757
GGGCTTT43550.017.20088860
GCGGGAC27100.017.1791572
ATGCGTG47950.016.9301328
TCCCCCG26200.016.83349670
CGTTTAT27350.016.7600359
TACGACT14300.016.6447756
CGCATAG22850.016.544365
GTCCCCC26750.016.48749769
GAACCGC14900.016.44468168
CGGGACT27650.016.3305053
Produced by FastQC (version 0.11.5)