FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_ox7.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_ox7.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9090258
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGC149570.16453878426772928No Hit
GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG136880.15057878445254247No Hit
ATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTGGTCATCGCG103720.11410017185430821No Hit
CTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCC101920.11212003003655122No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTTGGGAAG97060.10677364712860735No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG95640.10521153525015461No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA93750.10313238634150979No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC92600.10186729573572059No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA92340.10158127525093347No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA91680.10085522325108925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG13200.023.0670573
GTTACGA13850.021.4790954
TTACGAC14400.020.6587125
GTCCGTT5000.019.6004451
TCGTATG7350.018.09734343
TCGGATG37050.017.7589172
CATGCGT55750.017.3848867
ACATGCG58000.017.0767546
ATGCGTG57300.016.9754928
TATGCCG7800.016.60623246
CGGGACT27450.016.4472853
TGCGTGC61700.016.3887129
CTCGTAT8000.016.18902642
CGCATAG24700.016.15616865
TAAGTCG49100.016.104949
GTCCCCC28400.016.0234369
CGGAATA40100.015.97468968
CGCGGTT11950.015.8162581
TGCGAGT61500.015.81618913
GCGGGAC28550.015.8135882
Produced by FastQC (version 0.11.5)