FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_ox5.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_ox5.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7704613
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGC90620.11761784790488504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA28600.017.2535527
CGCTATG30050.016.30476629
AACGCTT31050.016.23155236
GAACGCT32400.015.66295535
TAAGTCG27000.015.5516039
GCAACGT32900.015.317245522
CCTATTA28600.014.80393111
ACGCTTG33850.014.78580537
TAGTCAT22750.014.4578659
GTCCCTA29650.014.3976918
TATCCCT35400.014.23995560
CATGCGT31900.014.040327
TAGGACC30750.013.7746749
CGTTAAA13800.013.69475133
TTAAGTC30150.013.6946968
GGGCTTT30950.013.68592460
ATGCGTG33150.013.616458
CGGAATA25200.013.61233668
GACTGTC31900.013.6050034
TCGGATG25300.013.5574762
Produced by FastQC (version 0.11.5)