FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_ox5.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_ox5.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8168581
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGC216850.2654683842885319No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTTGGGAAG169410.20739220190140734No Hit
CTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCC149280.1827489988775284No Hit
GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG146410.17923553674744733No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA146290.1790886324075136No Hit
CCCGGATCGGCCCGAGGGACTCGGGCCTTAGAGCCAAAAGGAGGGGCCAG140670.17220861248728514No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA133390.16329641586464036No Hit
GCCAGAGGCGCGCGGTGACCGGCTGCGCCGACGACGGCTGCCGGGGGCGC124960.15297638598429766No Hit
CTTTTCTGCTTAACGGCCCGCCAACCCTGGAAACGGTTCAGCCGGAGGTA124400.15229083239794036No Hit
GCGGTTATGAGTACGACCGGGCGTGGACGGTACTCGGTCCTCCGGATTTT122470.14992812093067326No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT121130.1482876891347469No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG114910.14067314751484988No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGC114820.1405629692598996No Hit
CGTTATTTTACTTATTCCGTGAGTCGGAAGCGGGGCCTGGCCCCTCCTTT111570.13658431005336177No Hit
CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGG111180.13610687094857724No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA109590.13416038844445566No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA108140.1323852943369234No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA107140.1311610915041425No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC105320.12893304234848135No Hit
GCCGTTCACATGGAACCTTTCTCCTCTTCGGCCTTCAAAGTTCTCATTTG98620.1207308833688495No Hit
CCGGAAGCCTGGTTACGGTGCCGAACTGCGCGCTAACCTAGAACCCACAA96670.1183436878449268No Hit
GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGAGTCGGAA94230.11535663293294149No Hit
CCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAAAG92750.11354481274042577No Hit
CCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCT91180.11162281429295982No Hit
GTCGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGC91160.11159833023630421No Hit
GCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGCCAGAGGCGCGCGGTGACC89150.10913768254241465No Hit
GTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGA87950.10766863914307762No Hit
CGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATT87540.10716671598163745No Hit
GCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGT84210.10309012054847715No Hit
CCCTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCC84160.1030289104068381No Hit
CCGGGAGATGCCCAGCTTGAGAATCGGGCGGCCGCGCCGTCCGAATTGTA83950.10277182781195413No Hit
GTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCCCGA83550.10228214667884178No Hit
GTGGTATTTCACTTGCGCCCGAGGGCTCCCACTTATCCTACACCTCTCAA83340.10202506408395778No Hit
AAAGGATTCAGCCCGCCGCCCGTTGGGAAGGGAGCTTCGAGGCGGCCGGC82310.1007641351661935No Hit
GTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGT82110.10051929459963732No Hit
ATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTGGTCATCGCG81830.10017651780645867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT7500.046.67511
GTCCGTT2550.037.0655171
CCTGCCA14850.033.9455261
GTGCGAC18050.029.0856083
CTGCCAG17950.027.6877672
GCGCTAT1850.026.4912721
CCCCCTT10250.025.9559061
TCAGGCG11200.025.3122652
GTCAGGC12200.024.1027151
TGCGACG22200.023.6484324
TGCCAGT21400.023.2240853
TGTTACG13050.023.0649223
GTCCCAT3650.023.0178551
TGGTCGG33700.022.53964670
CGGAAAC5450.022.4811251
GTTACGA13500.022.0368354
TTACGAC14100.021.0990985
GCGGTAT1506.384089E-721.0037941
CCCCGCA2900.020.5209481
CCGGCAT7700.020.458241
Produced by FastQC (version 0.11.5)