FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_ChiP-zmm16.shoot-line15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_ChiP-zmm16.shoot-line15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7400711
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC2317863.1319423228389813TruSeq Adapter, Index 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG156760.21181748618477333No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATG89310.12067759435546124TruSeq Adapter, Index 15 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT278250.061.6149142
TCGTATG285400.061.323143
CGTATGC291150.060.82380344
TCTCGTA277450.060.6693541
TATGCCG294850.060.10062846
GTATGCC297850.059.6825245
AAAAGGG290600.059.58980670
CCGTCTT293500.058.99387750
ATCTCGT290050.057.9851640
GTCACAT315350.057.58792529
GCCGTCT299200.057.57826649
ACATGAG319050.057.16411232
ATGCCGT312550.056.4510447
GAGCATC306300.056.3217236
CAGTCAC322350.056.30327627
GTCTTCT303400.056.2954452
CACATGA323700.056.2232431
CGTCTTC305900.056.21453551
TCACATG324050.056.10661730
AGTCACA325400.055.87384828
Produced by FastQC (version 0.11.5)