FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_ChiP-zmm16.shoot-line14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_ChiP-zmm16.shoot-line14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9171791
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC944731.0300387350736624TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC127600.056.8952344
CTCGTAT124850.056.8004942
ACGGTAG128850.056.41431832
TCGTATG129250.056.27631843
CACGGTA130450.055.69494231
TATGCCG132450.054.62895646
AGTCACG135500.053.67088728
TCTCGTA129150.053.66204541
GTATGCC136600.053.12246745
GGTAGCA137200.052.675734
CGGTAGC137650.052.63033733
GTAGCAT136350.051.82364335
TAGCATC134650.051.51706736
CCGTCTT141650.050.61187750
CAGTCAC147000.049.4954127
GCCGTCT145800.049.10032749
ATGCCGT148000.048.86663447
TCACGGT149800.048.73459630
GTCACGG149750.048.65765429
CCAGTCA151850.047.84541726
Produced by FastQC (version 0.11.5)