FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_ChiP-zmm16.shoot-line12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_ChiP-zmm16.shoot-line12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8678347
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT1550841.787022344232145TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC191050.060.30060646
TACGTAA193500.059.6896136
CTCGTAT187750.059.4003744
TATGCCG193700.059.3866548
ACGTAAT193250.059.17052537
GTACGTA195800.059.09468535
CCGTACG195800.058.8430833
TCGTATG196850.058.38096645
CGTAATC190150.058.20312538
ACCGTAC198350.058.15448832
ATGCCGT198100.058.0339449
GCCGTCT198100.057.94624751
CACCGTA201000.057.3706931
CCGTCTT200300.057.3097952
TCTCGTA193350.057.24380543
GTATGCC201200.057.22413347
CGTACGT204000.056.5124634
AATCTCG199600.055.4500741
TGCCGTC208700.055.13636850
TAATCTC201100.054.91463540
Produced by FastQC (version 0.11.5)