FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_ChiP-zmm16.root-line15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_ChiP-zmm16.root-line15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7465580
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT519330.695632489371221TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT69900.053.98531344
CGTATGC71550.053.57388346
TATCTCG72950.052.15624241
TCGTATG74400.051.56835645
TATGCCG75000.050.68940448
TCTCGTA76700.049.33473243
ATGCCGT77900.048.9375249
CACACTG78950.048.67399631
GTATGCC79550.047.8341447
ACTGATA81650.047.1946234
ACACTGA82550.046.76330632
CACTGAT84050.045.97192433
CAGTCAC84550.045.73900627
CTGATAT85350.045.35371835
GCCGTCT84050.045.3147751
GTCACAC85900.044.94019329
CCGTCTT85150.044.811352
TCACACT88150.044.1504430
CCAGTCA89000.043.49079526
TCCAGTC89650.043.48689325
Produced by FastQC (version 0.11.5)