FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_ChiP-zmm16.root-line14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_ChiP-zmm16.root-line14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7395863
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT333080.450359883626833TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130730.17676098110524763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT50550.049.99692544
TATCTCG50950.049.3946241
CGTATGC51600.049.1853946
TCGTATG53350.047.8991145
TATGCCG55150.045.9564848
AGTCACG55800.045.4076328
TCACGAG56550.044.93039330
ACGAGTG57350.044.30423732
TCTCGTA57050.044.29931643
CACGAGT58300.043.5820131
GGATATC57700.043.43129738
GTCACGA59250.042.8826429
GTATGCC59600.042.5245947
GTGGATA61400.041.26956636
CAGTCAC63850.040.01137527
GCCGTCT63350.040.00653551
ATGCCGT63900.039.882449
CCGTCTT63900.039.60687652
GATATCT64600.039.06535739
CGAGTGG65550.038.92294733
Produced by FastQC (version 0.11.5)