FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_ChiP-zmm16.root-line14-input1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_ChiP-zmm16.root-line14-input1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7393145
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC12550.010.32107246
ATGCCGT13450.08.84977549
CACGTCT68550.08.22125670
TATGCCG14800.08.04242348
CTCGTAT14900.07.987744344
TATCTCG16000.07.43798341
TCGTATG17950.07.215831845
ACACGTC76400.07.19326569
GCACACG94750.07.093192670
ACGTCTG58800.06.84605370
TCTCGTA21100.06.8016243
CGTCTGA57750.06.606847369
GTCTGAA64450.06.46314470
CACACGT86700.06.338701769
GATCGGA239500.05.758514470
AGCACAC123750.05.657239470
TCTGAAC55650.05.598150770
ATCGGAA216200.05.52100970
GAGCACA137250.05.508852570
CGGAAGA209550.05.49576370
Produced by FastQC (version 0.11.5)