FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_ChiP-zmm16.root-line12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_ChiP-zmm16.root-line12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6758863
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1114261.6485908946519554TruSeq Adapter, Index 21 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG282500.4179697088104907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC138450.060.1272746
GTCACGT144250.059.75522229
CTCGTAT134750.059.72370544
ACGTTTC144450.059.576932
AGTCACG145250.059.44020528
CACGTTT145850.059.09969331
TCGTATG140250.058.88090545
TCACGTT147350.058.54600530
TATGCCG142350.058.43335748
CGTTTCG148050.058.10544233
ATGCCGT144700.057.1940549
GTATGCC147000.056.9651647
TCTCGTA141450.056.74379343
GTTTCGG151900.056.58664734
GCCGTCT144650.056.34045851
ACGTCTG154700.056.07199515
CCGTCTT146100.055.9725752
CAGTCAC154050.055.93030527
CGGAATC146300.055.83662438
AATCTCG145500.055.35515241
Produced by FastQC (version 0.11.5)