FastQCFastQC Report
Mon 15 Jun 2020
HCWY5BGXF_n01_ChiP-zmm16.root-line12-input2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWY5BGXF_n01_ChiP-zmm16.root-line12-input2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8324119
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13200.08.75159444
AGTCACG12600.08.33274828
TCACGTA13700.07.66374130
GTCACGT12505.456968E-127.559468729
CTCGTAT14950.07.49250842
ATGCCGT13901.8189894E-127.303619447
CACGTAG14550.07.21607531
CGTAGAG15750.07.110781733
TATGCCG15900.07.045392546
ACGTAGA16501.03682396E-106.151156432
AGATCGG64900.05.98689970
ACGTCTG18051.6734703E-105.81564715
TCGTATG20857.2759576E-125.708233443
GTATGCC24555.456968E-125.27600545
ATCGGAA57800.05.208278770
ACACGTC23101.2732926E-105.15217570
CGTCTGA23502.0918378E-105.062500516
TCTCGTA23702.6375346E-105.02161941