FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30673172
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3629381.1832424765198721No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387150.12621779058259772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT92450.09.3629851
GGGGAAT69950.07.79916051
GTTAAAA277100.07.5076461
CCGGAAT76750.07.4872611
GTGTAGA329600.07.4323583145
CGGAAAT159000.07.27401641
TGTAGGG587550.07.2499785145
GTGTAGG635300.07.1398597145
AGTGTAG337950.07.1195736140-144
TAGGGAA575600.07.0216293145
GTAGGGT41700.06.9728103145
CGGGAAT86650.06.967611
GAATTGA168350.06.95638564
GTTGAAT225850.06.892361
GATCCAC98500.06.89169455
GTAGATA147300.06.8102064145
GTAGGGA563800.06.717328145
CTGGAAT93300.06.62691741
GAGTGTA381300.06.5921245140-144
TCGGTGG229550.06.586732145
Produced by FastQC (version 0.11.5)