FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22380092
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA345040.1541727353042159No Hit
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT312980.13984750375467625No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC279140.12472692248092634No Hit
GAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTTA272280.12166169826290259No Hit
GTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTAATCTGTT253380.11321669276426567No Hit
GCATAATTTAATTTTGTAGTATATATATAATTGGGATTATGACCTCGATG225750.10087089901149647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG110750.018.528196145
CGGTGGG100250.018.371294145
TCGTGTT256500.013.0883255145
GTTAAAA451900.012.4153791
GAGTGTT165150.012.073817145
GGTCATA122150.011.9862244
CAAAAAT793050.011.70873451
GTAGATA239900.011.636461145
GTAGGGT49950.011.467852145
GGGGAAT144850.011.3597491
AAAGTTC505400.010.9284319
GGATAGT188050.010.8701911
GCCGTAT353400.010.730639145
ATTAAAG619350.010.3102716
TAATCCC139750.010.1658189
TGGTCGC557250.09.902049145
GTTTAAT586200.09.8430261
GGTCGCC535650.09.81403145
GAGGTCA142750.09.7488322
TTAAAGT603900.09.6859627
Produced by FastQC (version 0.11.5)