FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24212304
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT325520.1344440413436078No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA309800.127951474589118No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC271890.11229414598462005No Hit
GTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTAATCTGTT270380.11167049612461499No Hit
GAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTTA257150.10620633211940508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG111250.020.465216145
TCTCGGG123600.014.137913145
GGTCATA130450.013.8906354
GTTAAAA474100.012.8893591
GGGGAAT162950.012.72287751
GTAGATA263350.012.444912145
GAGTGTT181350.012.394552145
GTAGGGT55000.011.733134145
TCGTGTT272350.011.660936145
GTCATAA160550.011.5121635
TAATCCC154800.011.4248949
GGATAGT201650.011.107951
AGGTCAT157550.010.5352543
GAGGTCA158750.010.4100672
CAAAAAT817150.010.2282471
GGTCGCC575750.010.188289145
TCGGTGG846900.09.700285145
GCCGTAT382000.09.699387145
GTTTAAT606950.09.6501061
GTGGTCG675550.09.595481145
Produced by FastQC (version 0.11.5)