FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21892868
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGT52600.011.303813145
TCTCGGG128200.010.633266145
CGGTGGG107000.010.300461145
TCGTGTT273000.010.066382145
GGATAGT129100.09.7699841
GTAGATA194000.09.119792145
TAGATCT519850.08.801327145
GAGTGTT162800.08.640606145
ATCTCGG463700.08.537927145
GTGTAGA642850.08.414455145
CAAAAAT595250.08.3296651
AGTGTAG654700.08.328507140-144
TGTAGGG1234750.08.192039145
AGAGTGT855550.08.187072145
GTGTAGG1331250.07.8759966145
TAGGGAA1166550.07.8256283145
GTAGATC592700.07.7806134140-144
TCGGTGG406500.07.777191145
TGTAGAT695900.07.6896462145
CTCGGTG454750.07.653593145
Produced by FastQC (version 0.11.5)