FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21895259
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT138800.09.6617651
TCGTGTT248900.09.321729145
GTGTAGA559650.08.861576145
CGGTGGG97450.08.853939145
TGTAGGG1069500.08.670839145
GTTAAAA383450.08.4314121
AGAGTGT739400.08.3547125145
ATCTCGG400200.08.315857145
AGTGTAG565150.08.228757140-144
GTGTAGG1153750.08.188495145
CAAAAAT657900.08.0874271
GTAGGGT47100.08.004861145
TGTAGAT626550.07.973241145
TAGGGAA1021450.07.935898145
GTAGATA205000.07.8164544145
GAGTGTA651550.07.667265140-144
GGGGAAT117300.07.60118441
TAGATCT470350.07.5534663145
GTAGGGA1015850.07.5157127145
GGAAAAT501400.07.47446971
Produced by FastQC (version 0.11.5)