FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21679216
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT237370.10949196686817456No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA230980.10654444330459181No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT159500.014.6792481
GGTCATA114350.012.9305464
GTTAAAA405750.011.3978811
TAATCCC127900.011.3910939
CGGTGGG93900.010.964982145
GATAGTT211600.010.721552
GTCATAA144550.010.6302545
TAGTTAA221000.010.39656454
GAGGTCA140050.010.2473352
GTAGGGT44850.010.185053145
CAAAAAT693050.09.8211641
TCGTGTT248150.09.817709145
AGGTCAT139800.09.7989493
CTCCATT129900.09.59728056
TTAAAAG470950.09.4497972
GGGGAAT95400.09.11791
TTAAAGT454000.09.0683747
CCATTCA124850.08.940658
ATTAAAG522250.08.8685246
AAAGTTC444650.08.8189919
Produced by FastQC (version 0.11.5)