FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22304305
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT149800.013.3070651
TCGTGTT269600.011.134316145
GTAGGGT54200.010.702218145
GATAGTT197000.010.191612
CGGTGGG105850.010.069538145
GTTAAAA391650.09.8463411
TAGTTAA207100.09.5544774
GTGTAGA642250.09.31393145
GAGTGTT171650.09.293129145
GGGGAAT107900.08.9349381
TAGATCT515300.08.72398145
AGTGTAG663400.08.479181140-144
GTAGATA205350.08.4036145
AGAGTGT860850.08.3554145
TTAAAAG438550.08.2472782
TGTAGGG1257600.08.238968145
TCGGTGG407000.08.2127695145
TGTAGAT699850.08.205467145
ATCTCGG457750.08.205112145
GACCCTA115950.08.0640477
Produced by FastQC (version 0.11.5)