FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23816869
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG114350.010.018677145
GGGGAAT124200.09.1631421
GGATAGT145600.09.0610061
GTGTAGA627150.08.960242145
TCGTGTT283350.08.67493145
GGGAAAT261800.08.611071
TGTAGGG1214450.08.370625145
GGTCATA115250.08.3645124
GTTAAAA392450.08.293321
TAGGGAA1153700.08.283464145
AGTGTAG626650.08.261513140-144
CAAAAAT659150.08.24791
AGAGTGT839850.08.132773145
ATCTCGG462600.08.103533145
GGAAAAT545950.08.0859461
TAGATCT515050.08.080756145
TCGGTGG405400.08.012791145
GTGTAGG1308900.07.916173145
TAATCCC109300.07.89158069
GTAGATA209500.07.85654145
Produced by FastQC (version 0.11.5)