FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23525960
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT153350.011.9117821
GGTCATA112950.09.9463974
GTAGATA214950.09.44517145
GGGGAAT114000.09.3470041
GTGTAGA620600.09.300162145
GTTAAAA413150.09.0882861
TGTAGGG1190200.08.577721145
GATAGTT206700.08.5561242
CAAAAAT696500.08.4715581
TAATCCC129550.08.3363629
TAGTTAA216500.08.3360774
GTGTAGG1288400.08.329141145
AGAGTGT825500.08.151174145
AGTGTAG620700.08.114059140-144
GTAGGGA1142000.07.9746714145
TAGGGAA1137900.07.9524274145
ATCTCGG459100.07.9441953145
AGGTCAT131850.07.91597653
GACCCTA130700.07.8192777
TGTAGAT679200.07.8038497145
Produced by FastQC (version 0.11.5)