FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23492431
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT160500.011.2457241
TCGTGTT281200.010.004748145
CGGTGGG110500.09.711563145
GTTAAAA404200.09.6303271
GTGTAGA587450.08.862235145
CAAAAAT703100.08.5467411
GATAGTT209200.08.4538082
TAGGGAA1075050.08.3431635145
ATCTCGG440600.08.21194145
CCTAGTT48700.08.1858977
AGTGTAG593100.08.156679140-144
TAGTTAA218050.08.11064154
AGAGTGT788000.08.097411145
GTGTAGG1216550.08.087972145
TGTAGGG1122250.08.03103145
GGAAAAT539900.07.9213971
GGTCATA108200.07.90453344
TCGGTGG392250.07.8562574145
GGGGAAT107150.07.8474561
TTAAAGT460150.07.844457
Produced by FastQC (version 0.11.5)