FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23143498
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT150200.011.5829011
GTAGGGT51850.09.649232145
CGGTGGG104800.09.617139145
TCGTGTT282600.09.313799145
GTTAAAA406600.09.2706891
GGGGAAT111800.09.0774161
CAAAAAT689250.09.0342561
GTGTAGA570550.09.023126145
GATAGTT198050.08.4176072
TTAAAGT439400.08.3468287
TAGATCT471400.08.306088145
TAGGGAA1052100.08.30467145
AGAGTGT762200.08.257398145
ATTAAAG508750.08.2490366
AGTGTAG577150.08.166049140-144
GTGTAGG1186400.08.165217145
TGTAGGG1092000.08.067632145
TTAAAAG459600.07.99583052
ATCTCGG431950.07.9735646145
GGAAAAT531950.07.97188571
Produced by FastQC (version 0.11.5)