FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23144296
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG73550.023.364151145
TCGTGTT322750.017.657963145
GTAGGGT81450.016.11283145
GAGTGTT198200.014.962493145
GCGTCGG237350.013.930283145
AGAGCGG295650.013.782968145
CGGTGGG136050.012.684189145
TCTCGGG148650.012.6333685145
GTAGATG163750.012.044033145
GGTCATA111400.011.6463254
GTTAAAA416950.010.8310621
TAATCCC115750.010.7706899
GGATAGT172150.010.4847791
CAAAAAT719200.09.5649691
GGGGAAT109150.09.4968441
GTCGTGG330350.09.19653145
CGGAAGC293350.09.021756145
AGTGTAG557450.08.857793145
TTAAAAG478200.08.6850562
GTGTAGG1133850.08.5243025145
Produced by FastQC (version 0.11.5)