FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23506617
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT171350.011.8446271
GGTCATA110950.09.9295714
GTTAAAA424800.09.79433251
GATAGTT221500.09.0313622
CTCCATT137000.08.835136
TGTAGGG1065550.08.7237835145
CAAAAAT736700.08.6584371
TAGTTAA233850.08.6473114
GTGTAGA568900.08.603171145
CCATTCA120150.08.5058538
GGGGAAT108950.08.3828331
TTAAAAG474700.08.3824512
GGGAAAT261650.08.2831961
GTGTAGG1156350.08.233151145
CGGTGGG103800.08.172957145
TCGTGTT269000.08.086483145
AGTGTAG571800.08.029402140-144
TCGGTGG380900.08.014231145
AGAGTGT757900.07.9406652145
ATTAAAG546650.07.9288256
Produced by FastQC (version 0.11.5)