FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24711453
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT170750.012.3539561
GGTCATA115050.09.6388244
GTTAAAA437100.09.3037021
GTGTAGA589250.09.068946145
GATAGTT225700.09.0240782
CAAAAAT737150.08.8503491
TAGTTAA242300.08.7048014
GTAGGGT51500.08.447593145
CCATTCA124450.08.4449958
TGTAGGG1106700.08.432136145
TCGTGTT292800.08.320647145
AGTGTAG592600.08.234597145
AGAGTGT794250.08.143227145
GTGTAGG1198650.08.033312145
CTCCATT141050.08.0162916
TAGGGAA1066900.07.965129145
GGGGAAT117700.07.9448741
TGTAGAT652200.07.893442145
GTAGATA217600.07.7306857145
TCCATTC161250.07.68639567
Produced by FastQC (version 0.11.5)