FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23574570
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGT49300.011.766289145
TCGTGTT278500.09.685325145
GGATAGT142600.09.4039531
TGTAGGG1005550.08.725256145
GTGTAGA524600.08.666339145
CAAAAAT692750.08.3394991
AGTGTAG526800.08.217221145
TAGGGAA968750.08.210911145
GTGTAGG1089650.08.118377145
GGGGAAT106400.08.1070621
CGGCTCT140150.08.0684421
AGAGTGT700750.08.060663145
GAGTGTT176500.08.052074145
CTCCATT134750.07.9607396
GTTAAAA396750.07.94751
GTAGGGA954700.07.7621202145
CTCTCCA151450.07.7528874
TAGATCT429750.07.727624145
CCATTCA131500.07.71668968
AAGAGTG782250.07.5082006145
Produced by FastQC (version 0.11.5)